Bio-Safety Laboratory Manufacture in Kolkata, India

Biosafety Laboratory Design & Installation Solutions

When it is comes to the best Bio-Safety Laboratory Manufacture in Kolkata, Cadillac Filters Pvt. Ltd is the best choice for you. With 45 years of experiences Cadillac Project specializes in the design, engineering, and installation of biosafety laboratories that ensure maximum containment, personnel safety, and regulatory compliance. Our biosafety lab solutions are developed to meet international standards while supporting pharmaceutical, diagnostic, research, and healthcare environments.

What Is a Biosafety Laboratory?

A Biosafety Laboratory is a specialized facility designed to safely handle and study biological agents, microbes, and infectious materials. These labs are built with specific containment measures, safety equipment, and protocols to protect laboratory personnel, the environment, and the surrounding community from potential hazards. Biosafety laboratories are classified into four levels—BSL-1 to BSL-4—based on the risk associated with the microorganisms being studied. Each level requires progressively stricter safety practices, specialized equipment, and controlled access to ensure research can be conducted safely and effectively.

Biosafety Levels (BSL-1 to BSL-4)

BSL-1 (Basic Level) – Low-risk microbes posing minimal threat. Necessary for basic research on nonpathogenic organisms. Elements: standard lab practices, PPE (gloves, lab coat, goggles), biohazard signs, safe handling of sharps, routine decontamination.

BSL-2 (Containment Level 2) – Moderate-risk agents that can cause human disease. Necessary to protect personnel from exposure to pathogens like HIV or Staph. Elements: PPE, biosafety cabinets for aerosols, self-closing doors, autoclave decontamination, restricted access, eye-wash stations.

BSL-3 (High Containment) – Serious or potentially lethal pathogens via inhalation. Necessary to prevent airborne transmission of agents like TB or West Nile Virus. Elements: respirators, solid-front gowns, controlled airflow, hands-free sinks, locked doors, medical surveillance, immunizations.

BSL-4 (Maximum Containment) – Extremely high-risk, deadly, exotic pathogens like Ebola. Necessary to protect staff and environment from fatal infections. Elements: full-body positive pressure suits, isolated facility, dedicated airflow, autoclave decontamination, Class III safety cabinets, strict entry/exit protocols.

Design Standards & Regulatory Compliance

All biosafety laboratories are designed following international and national guidelines, including:

• 1. WHO Biosafety Laboratory Guidelines
• 2. ISO 14644 Cleanroom Standards
• 3. GMP requirements (where applicable)
• 4. Indian biosafety and laboratory safety principles

Our designs focus on risk mitigation, containment, and long-term compliance, helping clients meet audit and approval requirements with confidence.

Technical Features of Our Biosafety Laboratories

Our biosafety lab solutions integrate advanced engineering systems to ensure safety and performance:

• 1. Custom Designs: Labs tailored for BSL-1 to BSL-4.
• 2. Advanced Filtration: HEPA & multi-stage air filters.
• 3. Energy-Efficient: Low-noise, optimized HVAC systems.
• 4. Safety Equipment: Biosafety cabinets, autoclaves, controlled airflow.
• 5. Durable Construction: High-quality, long-lasting materials
• 6. Access Control: Secure doors and restricted entry.
• 7. Monitoring Systems: Airflow, pressure, and safety sensors.

Why Choose Cadillac Project for Biosafety Laboratories?

• a. 45 Years of Hands-On Experience: Trusted expertise in delivering high-quality biosafety labs.
• b. Professional Technical Team: Skilled engineers and experts ensuring precision and safety.
• c. Custom Solutions: Tailored designs for BSL-1 to BSL-4 labs to meet specific client needs.
• d. Advanced Safety Systems: State-of-the-art filtration, controlled airflow, and strict protocols.
• e. Durable Construction: High-quality materials for long-lasting, reliable laboratories.
• f. Energy-Efficient Operations: Optimized HVAC and low-noise performance.
• g. Reliable Support: Comprehensive after-installation services and maintenance.
• h. Regulatory Compliance: Labs designed to meet international biosafety standards.

Our National and International Clients

Biosafety Level 1 (BSL-1)

The lowest of the four biosafety levels, biosafety level 1 (BSL-1) applies to laboratory settings in which personnel work with low-risk microbes that pose little to no threat of infection in healthy adults — for example, a BSL-1 laboratory might work with a nonpathogenic strain of E.coli. BSL-1 labs typically conduct research on benches, do not use special contaminant equipment, and do not need to be isolated from surrounding facilities.

Safety protocols for biosafety level 1 labs — which require only standard microbial practices — include:

• Mechanical pipetting (no mouth pipetting allowed)

• Safe sharps handling

• Avoidance of splashes or aerosols

• Daily decontamination of all work surfaces when work is complete

• Regular handwashing

• Prohibition of food, drink, and smoking materials

• The use of personal protective equipment (PPE), such as goggles, gloves, and a lab coat or gown

• Biohazard signs

BSL-1 labs also require immediate decontamination after spills. Infectious materials should also be decontaminated prior to disposal, generally through the use of an autoclave.

Biosafety Level 2 (BSL-2)

Biosafety level 2 (BSL-2) covers all laboratories that work with agents associated with human diseases — that is, pathogenic or infectious organisms — that pose a moderate health hazard. Common examples of agents found in a BSL-2 lab include equine encephalitis viruses, HIV, and staphylococcus aureus (staph infections). BSL-2 labs are required to maintain the same standard microbial practices as BSL-1 labs, as well as enhanced measures due to the potential risk the aforementioned microbes pose. Personnel working in biosafety level 2 laboratories are expected to take even greater care to prevent injuries, such as cuts and other breakage to the skin, as well as ingestion and mucous membrane exposures.

In addition to the safety protocols established for BSL-1 labs, BSL-2 labs are subject to the following safety controls:

• The use of PPE, including lab coats, gloves, eye protection, and — in some cases — face shields

• All procedures that could cause infection from aerosols or splashes must be performed within a biological safety cabinet

• Decontamination of infectious materials prior to disposal, generally through the use of an autoclave

• Self-closing, lockable doors

• Access to a sink and eyewash station

• Biohazard warning signs

Access to a biosafety level 2 lab is far more restrictive than to a biosafety level 1 lab. Outside personnel, or those with an increased risk of contamination, are often restricted from entering the area while work is underway.

Biosafety Level 3 (BSL-3)

Once again building on the two prior biosafety levels, a biosafety level 3 (BSL-3) laboratory typically conducts research into or work on microbes that are either indigenous or exotic and can cause serious or potentially lethal disease through inhalation. Common examples of microbes found in BSL-3 labs include yellow fever, West Nile virus, and the bacteria that causes tuberculosis. Microbes found within biosafety level 3 settings are so serious that work is often strictly controlled and registered through the appropriate government agencies. Laboratory personnel are also under medical surveillance and may require immunizations for the microbes they work with.

Common safety controls within a BSL-3 lab include:

• The use of PPE, including goggles and gloves; respirators may also be required

• The use of solid-front wraparound gowns, scrub suits, and/or coveralls is often required

• Access to a hands-free sink and eyewash station available near the exit

• Sustained directional airflow to draw air into the laboratory from clean areas toward potentially contaminated areas (exhaust air cannot be recirculated)

• Self-closing set of locking doors with access away from general building corridors

Access to a BSL-3 laboratory is restricted and controlled at all times.

Biosafety Level 4 (BSL-4)

Biosafety level 4 (BSL-4) labs are rare; however, a small number exist in the U.S. and around the world. As the highest level of biological safety, BSL-4 labs work with highly dangerous and exotic microbes, such as the Ebola and Marburg viruses. Infections caused by these types of microbes are often fatal and come without treatment or vaccines.

In addition to biosafety level 3 considerations, biosafety level 4 laboratories must follow these safety protocols:

• Personnel must change clothing before entering the facility and shower upon exiting

• All materials must be decontaminated before leaving the facility

• Personnel must wear the PPE from lower BSL levels, as well as a full-body, air-supplied, positive pressure suit

• Access to a Class III biological safety cabinet

BSL-4 labs are extremely isolated, often located in an isolated and restricted zone of a building or in a separate building entirely. BSL-4 labs also feature a dedicated supply of exhaust air, as well as vacuum lines and decontamination systems.